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Spreaders. Spreading colonies tend to be of three unique sorts: 1) a chain of colonies, not as well distinctly divided, that seems being due to disintegration of a bacterial clump; 2) one which develops in movie of drinking water among agar and bottom of dish; and three) one that forms in movie of drinking water at edge or on surface area of agar. If plates ready from sample have too much spreader expansion to ensure (a) location covered by spreaders, together with overall spot of repressed progress, exceeds fifty% of plate place, or (b) region of repressed advancement exceeds twenty five% of plate location, report plates as spreaders.
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